منابع مشابه
Tandem Mass Spectrometry and Glycoproteins
Glycoproteins and glycopeptides are organic compounds that are composed of both polypeptide and carbohydrate chains bonded together. For many years glycoproteins and glycopeptides have been a subject of interest; however, recently they have aroused the interest of biochemists and biologists from a wide range of fields. This increased interest is partly due to the fact that glycoproteins were di...
متن کاملStructure Determination of Glycoproteins by Mass Spectrometry
The relevant background information about the glycoprotein, enzymatic digestion, and the new matrix-assisted laser desorption/ionization time-of flight mass spectrometry (MALDITOF MS) ionization technique is reviewed. This method allows the detection of a complex mixture over a wide mass range (500-200,000 Da) using picomole to femtomole amounts of sample. Therefore, it is now possible to deter...
متن کاملAnalysis of the dispersity in carbohydrate loading of synthetic glycoproteins using MALDI-TOF mass spectrometry.
Statistical correlation of mass spectrum peak broadening with product dispersity in protein conjugation reactions allows more detailed characterization of putative therapeutic conjugates.
متن کاملMultiple-reaction monitoring liquid chromatography mass spectrometry for monosaccharide compositional analysis of glycoproteins.
A simple, sensitive, and rapid quantitative LC-MS/MS assay was designed for the simultaneous quantification of free and glycoprotein bound monosaccharides using a multiple reaction monitoring (MRM) approach. This study represents the first example of using LC-MS/MS methods to simultaneously quantify all common glycoprotein monosaccharides, including neutral and acidic monosaccharides. Sialic ac...
متن کاملN-glycosylation site occupancy in serum glycoproteins using multiple reaction monitoring liquid chromatography-mass spectrometry.
Congenital disorders of glycosylation (CDGs) are a family of N-linked glycosylation defects associated with severe clinical manifestations. In CDG type-I, deficiency of lipid-linked oligosaccharide assembly leads to the underoccupancy of N-glycosylation sites on glycoproteins. Although the level of residual glycosylation activity is known to correlate with the clinical phenotype linked to indiv...
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ژورنال
عنوان ژورنال: Trends in Glycoscience and Glycotechnology
سال: 2005
ISSN: 0915-7352,1883-2113
DOI: 10.4052/tigg.17.193